Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)

Formaldehyde in the fish body has been occured naturally through the work of trimethylamine-NOxide demethylase (TMAO)ase enzyme that breaked TMAO into formaldehyde and dimethylamine. Formaldehyde fish has been occured during storage. This reserch aimed to determine of TMAOase activity and its role in the formation of dimethylamine and formaldehyde lizard fish (Saurida tumbil) stored at chilling temperature. Observations were made every 2 days for 16 days of storage. Analysis was performed among other content of formaldehyde, dimethylamine, and specific activity of TMAOase enzyme. Result of formaldehyde test increased during storage of day 0 was 0.22±0.00 ppm to 7.45±0.46 ppm on day 16. The results of dimethylamine test increased during storage from 0,93±0,50 ppm at day 0 to 27.26±1.8 ppm on day 16. The results of specific activity TMAOase lizardfish activity increased during storage that was 1.15 U/ mg on day 0, to 16.47 U/mg on day 16.