VCA-IgA and Rta-IgG joint detection diagnosis and effectiveness of nasopharyngeal carcinoma

Objective To investigate the clinical efficacy of combined detection of VCA-IgA and Rta-IgG in the diagnosis of nasopharyngeal carcinoma. Methods From May 2013 to November 2014, 3 913 serum samples(male 2 367, female 1 546)from healthy people who had health examination in our medical center were collected and 169 serum samples(male 118, female 51) were collected from the patients who were diagnosed as nasopharyngeal carcinoma by pathological biopsy. Serum samples in two groups were detected by EBV RTA-IgG, VCA-IgA assay (ELISA) respectively. SPSS17.0 statistical software and receiver operating characteristic curve (ROC) were applied to data analysis. Results The Rta-IgG positive rates of EB virus were 93.5% in NPC group (158/169) and 2.4% (93/3 913) in healthy group; while the VCA-IgA positive rates were 79.3% in NPC group (134/169) and 8.9% (349/3 913)in healthy group. The sensitivity(χ2=14.49, P<0.05) and specificity(χ2=157.15, P<0.05) of Rta-IgG in the diagnosis of nasopharyngeal carcinoma were significantly better than that of VCA-IgA. Using VCA-IgA/Rta-IgG combined detection analysis, not only failed to effectively improve the diagnosis of nasopharyngeal cancer, but to reduce the detection sensitivity to 72.8%(123/169), compared with Rta-IgG detection only. Conclusions Rta-IgG is significantly better than that of VCA-IgA. There was no significant improvement in the clinical diagnostic efficacy of nasopharyngeal carcinoma using VCA-IgA/Rta-IgG combined detection mode.(Chin J Lab Med, 2016, 39: 609-612) Key words: Nasopharyngeal neoplasms; Herpesvirus 4, human; Immunoglobulin A; Immunoglobulin G; Capsid proteins; Enzyme-linked Immunosorbent assay