间充质干细胞
自愈水凝胶
再生医学
细胞生物学
巨噬细胞
组织工程
材料科学
脚手架
再生(生物学)
体内
免疫系统
干细胞
生物医学工程
纳米技术
体外
化学
免疫学
生物
生物化学
医学
生物技术
高分子化学
作者
Yiming Niu,Qiu Li,Ruiyu Xie,Shang Liu,Ruibing Wang,Panfei Xing,Yundi Shi,Ying Wang,Lei Dong,Chunming Wang
出处
期刊:Biomaterials
[Elsevier]
日期:2017-09-01
卷期号:139: 39-55
被引量:66
标识
DOI:10.1016/j.biomaterials.2017.05.042
摘要
The biomaterials-host interaction is a dynamic process in which macrophages play a vital role of regulation. Depending on the biochemical signals they sense, these highly plastic cells can mediate the immune response against the implanted scaffolds and/or exert regenerative potency to varying extent. Designing appropriate 'exterior signals' for scaffolds may exploit the power of endogenous macrophages to aid the regeneration of engineered tissues. To realise this goal, this study devised an injectable, instantaneously-solidifying coating material (acBSP) based on a unique, macrophage-affinitive glucomannan polysaccharide. Coating of three-dimensional hydrogel constructs with acBSP was rapid, neat and complete, requiring neither chemical reactions nor harsh conditions. Comprehensive in vitro analyses indicated that acBSP efficiently facilitated the adhesion and activation of macrophages and notably induced the macrophages to express pro-osteogenic/-angiogenic genes. Further in vivo assessment of acBSP-coated, mesenchymal stem cells-laden hydrogels in a murine dorsal subcutaneous pocket model demonstrated efficient macrophage activation, desirable scaffold-tissue integration and improved osteogenic differentiation in the delivered cells. In summary, by activating macrophages into a pro-osteogenic phenotype, the acBSP coating has demonstrated its competency as an innovative, open and efficacious platform to harness the power of host immunity for enhancing the regenerative performance of engineered tissue constructs.
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