DNA条形码
生物
内转录区
条形码
DNA测序
鉴定(生物学)
核糖体DNA
传统医学
DNA
计算生物学
核糖体RNA
系统发育树
植物
遗传学
GenBank公司
物种鉴定
进化生物学
基因
操作系统
计算机科学
作者
Dequan Zhang,Xin-Chun Mo,Jian-Ying Xiang,Nong Zhou
标识
DOI:10.21010/ajtcam.v13i6.12
摘要
DNA barcoding is a widely used tool that enables rapid and accurate identification of species based on standardized DNA regions.In this study, potential DNA barcodes, namely three plastid regions (rbcL, trnH-psbA and matK) and one nuclear ribosomal internal transcribed spacer (ITS) were adopted for species identification of original plants of Fritillariae Cirrhosae Bulbus.The rbcL and trnH-psbA regions showed better success rate of PCR amplification and DNA sequencing, as well as superior discriminatory ability. On the contrary, ITS region did not possess effective genetic variation and matK was faced with low success rate of sequencing. Combination of multi-loci sequences could improve identification ability of DNA barcoding. The trnH-psbA + rbcL could discriminate 25% - 100% species based on the Blast, Tree-Building and Distance methods.The potential DNA barcodes could not completely solving species identification of botanic origins of Fritillariae Cirrhosae Bulbus. In future, we should pay more attention to super-barcoding or specific barcode that enhance ability to discriminate the closely related plants.
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