溶血
抗氧化剂
抗坏血酸
化学
活性氧
脂质过氧化
槲皮素
生物化学
氧化应激
食品科学
药理学
生物
免疫学
作者
Thiago Mendanha Cruz,Amanda dos Santos Lima,Alessandra Oliveira Silva,Nima Mohammadi,Liang Zhang,Luciana Azevedo,Mariza Boscacci Marques,Daniel Granato
出处
期刊:Food Chemistry
[Elsevier]
日期:2024-05-01
卷期号:440: 138281-138281
被引量:1
标识
DOI:10.1016/j.foodchem.2023.138281
摘要
Oxidative/nitrosative damage takes part in chronic disease development, which generates an urgent need for intervention and better therapies to manage them. The scientific community has demanded easy-to-run, cheap, and reliable methods for cellular antioxidant activity assays. This work standardised and validated an erythrocyte cellular antioxidant activity and membrane protection/injury (HERYCA-P) protocol to study food-derive extracts. The method measures intracellular reactive oxygen species (ROS) generation, lipoperoxidation, and haemolysis induced by 2,2′-azobis(2-amidinopropane) dihydrochloride. Quercetin decreased ROS generation by 50.4% and haemolysis by 2.2%, while ascorbic acid inhibited lipid peroxidation by 40.1%. Total phenolic contents of teas were correlated with decreased ROS generation (r = −0.924), lipoperoxidation (r = −0.951), and haemolysis (r = −0.869). The erythrocyte ROS generation and lipoperoxidation were also associated with CUPRAC (r = −0.925; r = −0.951) and hydroxyl radical scavenging activity (r = −0.936; r = −0.949). The precision rates of antioxidant standards and tea samples were below 15%. HERYCA-P is feasible as a complementary antioxidant assay for food matrices.
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