肺炎克雷伯菌
肺炎
光动力疗法
光敏剂
医学
抗生素
肺炎克雷伯菌
炎症
免疫学
细菌性肺炎
免疫系统
微生物学
肿瘤坏死因子α
肺
金黄色葡萄球菌
细菌
内科学
生物
大肠杆菌
化学
生物化学
遗传学
有机化学
基因
作者
Tao Zhuo,Xin Li,Yu Heng,Junping Wu,Wen Ye,T F Liu
出处
期刊:ACS pharmacology & translational science
[American Chemical Society]
日期:2024-03-05
卷期号:7 (4): 1101-1113
标识
DOI:10.1021/acsptsci.3c00392
摘要
Klebsiella pneumoniae is a Gram-negative bacterium that induces acute lung injury (ALI) and inflammation in humans, necessitating immediate hospitalization and treatment. At present, the clinical treatment is largely dependent on hormones or antibiotics but is associated with drawbacks posed by the lack of eradication of the bacterium upon treatment and drug resistance. Therefore, there is an urgent need for novel and effective treatments. The current study investigated the treatment of K. pneumonia-induced ALI using a photosensitizer LD4 in conjunction with photodynamic therapy (PDT). The water content in the lungs (corresponding to edema) of a rat model of pneumonia induced by K. pneumoniae was reduced upon treatment with LD4-PDT. The counts of leukocyte, lymphocyte, and polymorphonuclear leukocyte in the blood were determined in the rat model of pneumonia, as were the concentrations of inflammatory cytokines (estimated using an enzyme-linked immunosorbent assay). The LD4-PDT treatment prominently reduced the levels of interleukin (IL)-6, IL-10, tumor necrosis factor-α, superoxide dismutase, and immune cells. Results suggest that LD4-PDT considerably alleviates the inflammation and oxidative stress caused by K. pneumoniae in the rat model of pneumonia. Furthermore, it could effectively improve the survival rate in the rat model of K. pneumonia-induced pneumonia and ameliorate histological changes while protecting the integrity of the pulmonary epithelial cells. These results highlight the potential application of LD4 as a photosensitizer for treating acute pneumonia induced by K. pneumoniae.
科研通智能强力驱动
Strongly Powered by AbleSci AI