单核细胞增生李斯特菌
生物膜
机制(生物学)
微生物学
化学
大蒜油
抗菌活性
食品科学
精油
生物
细菌
遗传学
认识论
哲学
作者
Miaomiao Liu,Wei Guo,Mingxing Feng,Yanhong Bai,Junrong Huang,Yungang Cao
标识
DOI:10.1016/j.lwt.2024.115847
摘要
Garlic essential oil (GEO) in water nanoemulsion (GEON) has been reported to possess good stability and broad-spectrum antibacterial activity, but its effect on Listeria monocytogenes is rarely studied. In the present study, the antibacterial, anti-biofilm formation activity and underlying mechanism of GEON against L. monocytogenes were investigated. Results showed that GEON (mean particle size = 136.43 nm) significantly inhibited the growth of planktonic L. monocytogenes, and the minimum inhibitory concentration (MIC) of GEO in the nanoemulsion was 512 μg/mL. GEON could effectively kill L. monocytogenes in a time- and concentration-dependent manner. After GEON treatment, the cell membrane was severely damaged, which was proven by remarkable membrane depolarization and considerable loss of intracellular substances (nucleic acid, protein, and ATP). Moreover, decreased membrane integrity and collapsed cell morphology further confirmed the cell membrane damage. Additionally, GEON significantly attenuated the biofilm formation, enhanced the surface hydrophobicity, and reduced the swimming motility of L. monocytogenes. These results indicate that GEON has the potential to inhibit the planktonic growth and biofilm formation of L. monocytogenes.
科研通智能强力驱动
Strongly Powered by AbleSci AI