Identification of anthocyanins in deep colored berries and grains in China

Identification of anthocyanin relies heavily on authentic anthocyanin standards and the detection instruments employed, and both of these made tremendous improvement in the past decades. Here, with 118 authentic anthocyanin standards and state-of-the-art detection method, we comprehensively analyze the anthocyanin composition of the most commonly seen, deep-colored berries and grains in China. Cyanidin-3-O-glucoside is the overwhelmingly major anthocyanin in all the berries and grains examined, including blue honeysuckle, blueberry, cranberry, elderberry, mulberry, black rice, and black soybean, which accounts for an average of 82% of the total anthocyanins with a little variation between them. Peonidin-3-O-glucoside is the second most abundant anthocyanin ranging from 2.6%–14.9% of the total anthocyanins. Pelargonidin-3,5-O-diglucoside is only found in blue honeysuckle, and besides that, berries and grains share a dominant portion of common anthocyanins among them. This study primes the survey of anthocyanin in common Chinese foods for the establishment of a nutrition database.