斑马鱼
生物
清脆的
达尼奥
DNA甲基化
基因组编辑
表观基因组
计算生物学
表观遗传学
甲基化
遗传学
DNA
Cas9
细胞生物学
基因
基因表达
作者
Fang Liang,Zijiong Dong,Jianmin Ye,Wei Hu,Ramji Kumar Bhandari,Kangsen Mai,Xuegeng Wang
出处
期刊:Epigenetics
[Informa]
日期:2023-03-22
卷期号:18 (1)
被引量:1
标识
DOI:10.1080/15592294.2023.2192326
摘要
The CRISPR/dCas9-based epigenome editing technique has driven much attention. Fused with a catalytic domain from Dnmt or Tet protein, the CRISPR/dCas9-DnmtCD or -TetCD systems possess the targeted DNA methylation editing ability and have established a series of in vitro and in vivo disease models. However, no publication has been reported on zebrafish (Danio rerio), an important animal model in biomedicine. The present study demonstrated that CRISPR/dCas9-Dnmt7 and -Tet2 catalytic domain fusions could site-specifically edit genomic DNA methylation in vivo in zebrafish and may serve as an efficient toolkit for DNA methylation editing in the zebrafish model.
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