Continuous-flow label-free size fractionation of extracellular vesicles through electrothermal fluid rolls and dielectrophoresis synergistically integrated in a microfluidic device

Effective separation of small extracellular vesicles (EVs) especially exosomes from large EVs with a high recovery rate and purity based on electrokinetic principles in a microfluidic device featuring three-dimensional silicon microelectrodes.