A hand-powered positive pressure-driven sample injection digital RPA-CRISPR-Cas12a chip

= 0.99973). The tests conducted on the milk samples containing the standard substances further proved that this method could be used for on-site testing under resource-limited conditions. Overall, this study provides a feasible strategy for implementing positive-pressure digitization in a portable chamber-based digital microfluidic system, which expands the utility of chamber-based digital assays for portable and instrument-free on-site food-safety testing.