甲基化
癌症研究
CpG站点
弥漫性大B细胞淋巴瘤
生物
淋巴瘤
DNA甲基化
表观遗传学
基因沉默
基因
基因表达
遗传学
免疫学
作者
Yiwen Zhang,Jie Zhang,Jun Li,Junfeng Zhu,Yanli Yang,Lili Zhou,Zhongli Hu,Feng Zhang
标识
DOI:10.3109/10428194.2014.994181
摘要
The positive regulatory domain 1 (PRDM1) exists as two isoforms: PRDM1α and PRDM1β. The former is frequently inactivated, while the latter is overexpressed in a subset of diffuse large B-cell lymphoma (DLBCL). To investigate the possible epigenetic alteration of PRDM1α and PRDM1β expression, the methylation of these two promoter isoforms was assessed in B lymphoma cell lines and DLBCL samples. Hypomethylation of PRDM1β CpG islands was preferentially detected in lymphoma cells. However, both high and low methylation of PRDM1α CpG islands was simultaneously observed in cases of DLBCL compared with the moderate methylation of non-tumor cases. CpG 16–21-specific high methylation was correlated with low expression of PRDM1α in PRDM1β-positive DLBCL samples. Three increased and one decreased miRNAs were significantly different between cases of DLBCL and non-tumor reactive hyperplasia. Thus, our results indicate that aberrant methylation silencing of PRDM1α and hypomethylation activation of PRDM1β are frequent events in DLBCL.
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