Methylated Bovine Serum Albumin as a Carrier for Oligo- and Poly-Nucleotides

We initiated an immunochemical study of DNA in 1956, at a time when there was little decisive data dealing with the antigenicity of DNA (Blix et al., 1954; Lackman et al., 1941) and no evidence that chemically pure DNA was capable of eliciting antibody formation in a suitable host. For this reason, we used in our initial experiments DNA-rich preparations extracted from Brucella abortus by a phenol procedure (Braun et al., 1957). These preparations contained appreciable protein and carbohydrate material that could not be dissociated easily from the DNA.