[Digital loop-mediated isothermal amplification detection technology and its application progress].

Digital loop-mediated isothermal amplification (digital LAMP, dLAMP) is a novel nucleic acid amplification technique developed in recent years. It divides the target nucleic acid and LAMP reagent into a large number of independent detection regions, and uses a highly active chain replacement DNA polymerase and four specially designed primers for rapid amplification under isothermal conditions, which provides a good platform for quantitative detection of target nucleic acids. The advantages of high accuracy, high sensitivity, absolute quantification, high tolerance to inhibitors and simple instrumentation make the dLAMP technique very promising in molecular diagnosis, especially in rapid detection of pathogenic microorganisms, it shows a good application prospect in the fields of clinical diagnosis, food safety and environmental monitoring. Certainly, the development of dLAMP still faces some challenges, such as how to avoid non-specific amplification in multiple primer designs, multi-target nucleic acids and simultaneous detection of a large number of samples. With the development of dLAMP technology, this technology will greatly enrich the future development of molecular diagnostics. Applying rapid and effective molecular diagnostic techniques to the diagnosis of pathogenic microorganisms has important social significance for disease prevention and control.数字环介导等温扩增（digital LAMP，dLAMP）是近年发展起来的一种新的核酸扩增技术，是将目标核酸和LAMP试剂分割成大量的、独立的检测区域，利用一种高度活性的链置换DNA聚合酶和4种特殊设计的引物在等温条件下进行快速扩增，其为目标核酸定量检测提供了一个良好的平台。dLAMP具有准确度高、灵敏度高、绝对定量、对抑制剂耐受性强以及仪器要求简单等特点，使得其在分子诊断中特别是病原微生物快速检测中极具潜力，在临床诊断、食品安全、环境监测等领域展现出良好的应用前景。同时，dLAMP的发展还面临一定的挑战，例如多种引物设计如何避免非特异性扩增、多目标核酸和超大量样本同时检测等。随着dLAMP技术的不断进展，这项技术必将极大地丰富分子诊断技术的未来发展方向。将快速、有效的分子诊断技术应用于病原微生物的诊断，对疾病的预防控制具有重要的社会意义。.