Bradford protein assay – Protein concentration measurement (A590/A450 improved linearity) v1

This protocol describes how to measure the concentration of total protein in a sample performing the Bradford's assay using microtiter plates. Procedures are slightly modifications based on the method described in (1) and the linearization procedure described in (2) and (3). (1) Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248–254. (2) Zor & Selinger, Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies, Anal. Biochem. 236 (1996) 302–308. (3) Ernst & Zor, Linearization of the Bradford protein assay, J. Vis. Exp. 38 (2010) 1918.