自愈水凝胶
生物制造
细胞外基质
材料科学
纳米技术
3D打印
生物加工
软机器人
模块化设计
组织工程
计算机科学
智能材料
仿生学
3D生物打印
生物医学工程
人工智能
化学
复合材料
工程类
生物化学
遗传学
高分子化学
执行机构
生物
操作系统
作者
Janko Kajtez,Milan Finn Wesseler,Marcella Birtele,Farinaz Riyahi Khorasgani,Daniella Rylander Ottosson,Arto Heiskanen,Tom Kamperman,Jeroen Leijten,Alberto Martı́nez-Serrano,Niels Bent Larsen,Thomas E. Angelini,Malin Parmar,Johan Lind,Jenny Emnéus
标识
DOI:10.1002/advs.202201392
摘要
Abstract Human in vitro models of neural tissue with tunable microenvironment and defined spatial arrangement are needed to facilitate studies of brain development and disease. Towards this end, embedded printing inside granular gels holds great promise as it allows precise patterning of extremely soft tissue constructs. However, granular printing support formulations are restricted to only a handful of materials. Therefore, there has been a need for novel materials that take advantage of versatile biomimicry of bulk hydrogels while providing high‐fidelity support for embedded printing akin to granular gels. To address this need, Authors present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human stem cells inside Self‐Healing Annealable Particle‐Extracellular matrix (SHAPE) composites. SHAPE composites consist of soft microgels immersed in viscous extracellular‐matrix solution to enable precise and programmable patterning of human stem cells and consequent generation mature subtype‐specific neurons that extend projections into the volume of the annealed support. The developed approach further allows multi‐ink deposition, live spatial and temporal monitoring of oxygen levels, as well as creation of vascular‐like channels. Due to its modularity and versatility, SHAPE biomanufacturing toolbox has potential to be used in applications beyond functional modeling of mechanically sensitive neural constructs.
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