Study on Compound Enzymatic Hydrolysis of Jew's-ear's Polysaccharides

[Objective] To optimize compound enzymatic hydrolysis of Jew's-ear's polysaccharides. [Method]With polysaccharides yield as indicator,effects of enzymatic temperature,solution p H,solid-liquid ratio,enzymatic time on yield of Jew's-ear's polysaccharides were investigated. TLC method was used to analyzed the compounds of Jew's-ear's polysaccharides. [Result] The experiments determine the optimum conditions for enzymatic hydrolysis of Jew's-ear's polysaccharides: leaching agent multiples is 1 to 40,p H is 7. 0,temperature is 40 ℃,time is 3. 0 h. Under this condition,Jew's-ear's polysaccharides extraction rate is 4. 353%. The monosaccharide composition of polysaccharides is D-glucose. [Conclusion] The study can provide reference basis for extraction of Jew's-ear's polysaccharides.