In this paper,outlined an early qualitative and quantitative monitoring of the Puccinia striiformis f.sp.Tritici,introduced the design and application of species specific primer of Puccinia striiformis f.sp.Tritici fungus,mainly includes the development of the specific markers of physiological races of Puccinia striiformis f.sp.Tritici races,and application of the specific primers of Puccinia striiformis race.