Determination of lobetyolin in Radix Codonopsis by high-performance liquid chromatography

OBJECTIVE To establish the quality evaluation system of lobetyolin in Radix Codonopsis.METHODS MetaChem RP-18(250 mm×4.6 mm,5 μm) was used with acetonitrile-water(22∶78) as the mobile phase and UV detection was at 267 nm.RESULTS 11 batches of Radix Codonopsis were analyzed.The content of lobetyolin ranged from 0.035～1.314 mg·g -1.CONCLUSION The method was simple,reproducible and reliable. It can be used to control the quality of Radix Codonopsis.