In vivo electroporation enhances foreign GRF gene expression efficiency in skeletal muscle of mice.

The GRF gene plasmids(pIRES-GRF and pcDNA3-GRF) were injected into skeletal muscle of mouse by electroporation in vivo.The dosage of injection was 5,10,50 μg GRF gene plasmid,respectively.The result showed that at 10 d postinjection,concentrations of serum GRF of mice in the group injected with 5 μg pGRF by electroporation in vivo was 44.73%(P0.05) higher than the control,and 12.86%(P0.05) higher than the group injected with 50 μg pGRF.At 30 d postinjection,accumulative increased body weights in the group injected with 5 μg pGRF by electroporation in vivo was 11.46%(P0.05) higher than the control and 6.75%(P0.05) higher than the group injected with 5 μg pGRF.The accumulative increased body weights in the group injected with 10 μg pGRF directly was 19.52%(P0.05) higher than the control,and 19.42%(P0.05) higher than the group injected with 10 μg pGRF by electroporation in vivo.After 45 d,accumulative increased body weights in the group injected with 50 μg pGRF directly was 14.00%(P0.05) higher than the control,and 12%(P0.05) higher than the group injected with 50 μg pGRF by electroporation in vivo.The results suggested that in vivo electroporation was able to enhance GRF expression in mouse skeletal muscle in a strict manner.