Objective At present dialysis machines provided online assessment of Kt/V.The aim of our study was to assess the effect of hemodialysis time on the rate of urea removal(K),by measuring K at different times of hemodialysis patients.Methods The K during one hemodialysis session were observed chronologically.The relationship between hemodialysis time and K were analyzed.Results There was no correlation between the hemodialysis time and K(P 0.05).Conclusion The rate of urea removal was uncorrelated with hemodialysis time during one hemodialysis session.Kt / V was positively correlated with hemodialysis time.