Cloning and Sequence Analysis of TUB Gene Fragment from Sedirea japonica

TUB of Sedirea japonica was cloned and sequence analyzed,and the result would provide an important basis for selecting a reference gene and studying the function of TUB. The total RNA of Sedirea japonica scapes was extracted by CTAB method and the partial sequence of Sedirea japonica TUB was cloned by RT-PCR,which was1 055 bp long and encoded 351 amino acids residues. Its amino acid sequence contained a conservative region GGGTGSG. The sequencing result revealed that the TUB gene fragment from Sedirea japonica homology comparison with other plants TUB gene sequences in the Gen Bank shows that they share 75%- 96% nucleotide sequence homology,96% nucleotide sequence homology with Doritaenopsis hybrid TUB,the amino acid sequence homology is above 96% and amino acid sequence homology high similarity with the monocotyledon. The cloned genes were TUB gene fragment,which were registered them into Gen Bank( respective accession number: KP686397).