The morphological and cytological observations were conducted on the male sterility induced by CHA GENESIS in wheat.The results indicated that spikes were light green in color and arrayed incompactly as the spike expanded which resulted in stamen degeneration. There were two types of abnormal anther and pollen development,anthers without pollen and anthers containing abortive, abnormal multicellular pollen. There was antherless pollen cell,and the pollen mother cell was disorganized when GENESIS was sprayed at the stage of Feek's 8.0. The pollen abortion existed, which stayed at mononuclear microspore stage and some reached binuclear microspore stage when GENESIS was sprayed at the stage of Feek's 9.0, but the microspore was in abnormal shape and the protoplasts of the pollen disappeared. The abortive pollen was more critical when treated with GENESIS at the earlier stage. The effect on the anthers was less significant at the dosage of 3 kg·hm-2 treated with GENESIS.