Effects of different ratios of Tannin and Stilbene Glucoside from Polygonum Multiflorum on liver biochemical indexes in rats

Objective To research the effects of different ratios of tannin and stilbene glucoside on liver biochemical indexes in rats and to explore the possible material basis of liver toxicity in Polygonum Multiflorum.Methods A total of 120 SD rats were randomly divided into 4 groups: the control group(group A) was given 1ml/100g of distilled water;group B was treated with tannin 4.4g·ml-1·kg-1,group C was given tannin(4.4g/kg) and stilbene glycosides(1.0g/kg) on 1∶1 ratio;group D was given tannin(4.4g/kg) and stilbene glycosides(1.0g/kg) on 2∶1 ratio.The rats were given the appropriate drugs.The rats were treated for 90 days continuously and 15d in recovery.The carotid artery blood samplings were taken at 60d and 90d and 15d in recovery period after administration,to detect the liver function by biochemical indicators.Results Compared with group A,administered for 60d,the ALT in treatment group was increased,the difference was statistically significant(P0.01);the CHE was increased and TBIL was decreased in group B,the difference was statistically significant(P0.01);the TP was increased in group B and group C,the difference was statistically significant(P0.01).Compared with group A,administered for 90d,the CHE and TP were increased but the LDH was decreased in group B,the difference was statistically significant(P0.01).The CHE,LDH and ALB were decreased in group C and group D,the difference was statistically significant(P0.01).Compared with group A,in recovery period,the TP and ALB were increased in group B,the CHE and ALP were decreased in group C;the CHE and LDH were decreased in group D,the difference was statistically significant(P0.01).Conclusion Long-term and large dosage of tannin and stilbene glycosides extracted from Polygonum Multiflorum with different ratios can damage the liver of rats,but the liver damage can be relieved after withdrawal.