The Pre-Screening for Taxol-produing Endophytic Fungi from Taxus

Objective: In order to get a high-taxol-yield endophytic fungi which could be fermented to produce taxol.Method: Endophytic Fungi were isolated from root,stem and leaf of different source taxus,and were fermented,and then,Taxol in mycelium and fermenting liquor was determined by HPLC.Result:54 strains of Taxol-produing endophytic fung were obtained,29 strains from root,and 16 strains from stem,and the other from leaf.The average yield of the taxol in both mycelium and fermenting of endophytic fungi from three parts were 248.57μg/L,149.09μg/L,104.94μg/L.The highest yield was 622.75μg/L.Conclusion:Through comparison,one strain of high-taxol-yield endophytic was gained which from root of wild Taxus.chinesis.