AFLP and SCAR markers for seed colour in rape Dahuang(Brassica rapa L.)

Rape Dahuang is a special landrace of Brassica rapa L.which originated from Qinghai-Tibetan Plateau with bright yellow seeds.Previous results showed the yellow-seeded trait in Dahuang was controlled by a recessive gene(Brsc1),which was mapped on A09 in B.rapa.For more specific markers linked to Brsc1,BC1population and F2population were constructed from the cross of Dahuang and 09A-126(brown-seeded,B.rapa).Amplified fragment length polymorphism(AFLP) technology combined with bulked segregant analysis(BSA)was conducted by screening 256 pairs of AFLP primers in BC1population,5 markers tightly linked to Brsc1 were obtained.5 specific AFLP fragments were cloned,sequenced and identified,and compared to that of synteny in B.rapa genome,showing homology to A09 of B.rapa.The AFLP markers were converted into 5 SCAR(sequence characterized amplified region) markers for F2population.One co-dominant marker was detected.