Study on Bone Marrow-derived Stromal Cells Enhancement of Hematopoiesis Reconstitution

Objective To investigate the role of bone marrow-derived stromal cells in hematopoietic reconstitution in vivo.Methods Stromal cells isolated from Balb/c mice were incubated in medium with bFGF.Cultured stromal cells plus whole BMCs were injected in vein into Balb/c mice irradiated lethally.Mortality rate and leukocyte were counted.Results More mice transplanted with BMCs plus stromal cells survived than those with BMCs only and hematopoietic recovery with BMCs plus stromal cells was rapid.MSCs can be transplantated i.v.without any toxicity.Conclusion Bone marrow-derived stromal cells can enhance hematopoitic reconstitution and can be transplanted in security.