化学
旁分泌信号
脂肪细胞
MAPK/ERK通路
脂解
炎症
NF-κB
巨噬细胞
细胞生物学
生物化学
内科学
脂肪组织
内分泌学
体外
磷酸化
生物
信号转导
医学
受体
作者
Qiaozhi Zhang,Diego A. Luna‐Vital,Elvira González de Mejía
标识
DOI:10.1016/j.jff.2019.01.016
摘要
This study aimed to investigate the anti-inflammatory effect of two anthocyanin-rich extracts from purple (PMW) and red maize (RMW), in comparison to three pure anthocyanins [cyanidin-3-O-glucoside (C3G); peonidin-3-O-glucoside (P3G); pelargonidin-3-O-glucoside (Pr3G)] on the inflammatory states that occurred during the macrophage-adipocyte interaction, using both mono- and co-culture in vitro models. PMW and RMW reduced production of pro-inflammatory cytokines (TNF-α: −8.8% to −44.2%; MCP-1: −18.4% to −56.9%) in both macrophages and adipocytes mono-cultures. PMW and RMW, as well as C3G and Pr3G inhibited activation of NF-κB and JNK pathways, via regulation of phosphorylation of IκBα and JNK, respectively. Both extracts and pure anthocyanins restored inflammation-mediated oxidative stress and insulin resistance in macrophage-conditioned media-treated adipocytes. PMW and RMW decreased pro-inflammatory cytokine production and lipolysis, while enhanced the glucose transporter 4 membrane translocation in the adipocyte-macrophage co-cultures. These results suggested that colored maize anthocyanins could potentially ameliorate the paracrine interplay between adipocytes and macrophages.
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