New Δ8,9-pregnene steroids isolated from the extremophile fungus Exophiala oligosperma

Three pregnene steroids, two of them new, were isolated from ethyl-acetate partition of liquid-cultivation of the extremophyle fungus Exophiala oligosperma, found in a pH 1.5 hydrochloric acid aqueous solution. Spectroscopic studies using NMR and HRMS, allowed the identification of the molecular structures of both Δ8,9-pregnenes, still not described in the literature.