药理学
氧化应激
医学
肾缺血
肾
血管内皮生长因子
肌酐
一氧化氮
丙二醛
内分泌学
内科学
缺血
再灌注损伤
血管内皮生长因子受体
作者
Amira Awadalla,Abdelaziz M. Hussein,Mohamed Ali,Nashwa Barakat,Eman T. Hamam,Raghda W. Magar,Ahmed A. Shokeir
出处
期刊:Life Sciences
[Elsevier]
日期:2021-05-01
卷期号:272: 119221-119221
被引量:10
标识
DOI:10.1016/j.lfs.2021.119221
摘要
The present study aimed to investigate the invitro preconditioning of adipose-derived mesenchymal stem cells (ADMSCs) with CD44-targeted hyalournic acid (HA) on ischemic kidney injury in rats. Ninety male Sprague Dawley rats were randomly allocated into the following groups; i) sham group, ii) control group: rats exposed to 45 min left renal ischemia with saline treatment, iii) HA group as control group but rats treated with HA, iv) ADMSCs group as control but rats treated with ADMSCs v) HA + ADMSCs group as ADMSCs but rats treated with ADMSCs preconditioned with CD44-tageted HA for 14 days. We found that treattment with either ADMSCs or HA + ADMSCs caused significant decrease in the elevated serum creatinine and BUN and malondialdehyde (MDA) concentrations and expression of TGF-β1, fibronectin, collagen type I, inducible nitric oxide synthease (iNOS) and microRNAs (miR-21, miR-17-5p, miR-10a) in kidney and significant increase in creatinine clearance, superoxide dismutase (SOD), reduced glutathione (GSH) and the expression of Bcl2, vascular endothelial growth factor (VEGF), Wnt/β-catenin pathway genes in kidney compared to control group (p < 0.05). Moreover, HA + ADMSCs group caused more significant improvement in these parameters than ADMSCs group (p < 0.05), while HA group did not cause any significant improvement in these parameters compared to control group. These results suggest that preconditioning of ADMSCs preconditioned with CD44-targted HA enhanced their cytoprotective effect against ischemic kidney injury. This renoprotective effect might be due to activation of angiogenesis, Wnt/β-catenin pathway proteins, and suppression of oxidative stress, apoptosis, inflammation and fibrosis.
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