化学
电穿孔
里氏木霉
转化(遗传学)
生物物理学
细胞生物学
转化效率
纤维素酶
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2234: 73-78
标识
DOI:10.1007/978-1-0716-1048-0_6
摘要
During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less expensive, and easier to perform than the classical protoplast transformation while at the same time having a comparable efficiency.
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