土霉素
检出限
拉曼光谱
四环素
表面增强拉曼光谱
拉曼散射
胶体金
自体荧光
色谱法
纳米技术
材料科学
化学
荧光
纳米颗粒
光学
物理
生物化学
抗生素
作者
Jing Qian,Changrui Xing,Yonghui Ge,Rui Li,Aitong Li,Wenjing Yan
标识
DOI:10.1016/j.saa.2020.118146
摘要
Rapid and accurate detection of antibiotics at trace levels in food represents a great challenge. Tetracycline (TC), as a sort of broad-spectrum antibiotic, has been extensively used in animal infection therapy and animal husbandry as growth promoters. Large amounts of TC residues in animal-derived foods affect food quality and safety, and cause undesirable side effects such as allergic reactions and bacterial antibiotic resistance. Here, a Raman fingerprint strip sensor was reported based on surface-enhanced Raman scattering technology and demonstrated for ultrasensitive detection of TC. In this approach, 4-aminothiophenol (4-ATP) modified gold nanostars (GNSs) were used as a strong Raman reporter, which was coated with anti-TC monoclonal antibody serving as a biorecognition to acquire both visual and Raman signals on the test line. To demonstrate the performance of this strip, TC standard solutions with concentrations ranging from 0.5 to 50 ng/mL was detected, the limit of the detection (LOD) for the Raman signal was 0.04 ng/mL, which was 100 times more sensitive than those of color intensity quantifications. The other analogues, oxytetracycline, and chlortetracycline were detected using this method, making them suitable for the samples with TC analogues screening.
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