细胞培养
真核生物翻译延伸因子1α1
细胞质
生物
肝细胞癌
细胞凋亡
基因表达
分子生物学
细胞分化
细胞
信使核糖核酸
细胞生物学
癌症研究
基因
遗传学
核糖体
核糖核酸
作者
Gabriele Grassi,Bruna Scaggiante,Rossella Farra,Barbara Dapas,Francesco Agostini,Bruno Daniele,Natalia Rosso,Claudio Tiribelli
出处
期刊:Biochimie
[Elsevier]
日期:2007-12-01
卷期号:89 (12): 1544-1552
被引量:66
标识
DOI:10.1016/j.biochi.2007.07.007
摘要
Despite the involvement of the elongation factors eEF1A (eEF1A1 and eEF1A2) in the development of different cancers no information is available on their possible contribution to the biology of hepatocellular carcinoma (HCC). We investigated the expression of both forms of eEF1A in HepG2 and JHH6 cell lines considered to be a good in vitro model of HCC at different stage of differentiation. Our data indicate that the mRNA amount of eEF1A1 is increased in both cell lines as compared to normal liver tissue, but eEF1A2 mRNA level is markedly increased only in JHH6. Moreover, the less differentiated cell line JHH6 displays higher EEF1A1 and EEF1A2 mRNAs levels and an higher nuclear-enriched/cytoplasm ratio of EEF1A protein compared to the better differentiated HepG2 cell line. Over-expression depends only partially on gene amplification. The more abundant mRNA levels and the higher nuclear-enriched/cytoplasm ratio of eEF1A in JHH6 neither correlate with apoptosis resistance nor with proliferation rate in sub-confluent cells. However, in confluent cells, a clear tendency to maintain JHH6 into the cell cycle was observed. In conclusion, we document the increased mRNA levels of EEF1A genes in HCC cell lines compared to normal liver. Additionally, we show the increased nuclear-enriched/cytoplasmic protein ratio of eEF1A and the marked raise of the expression of both eEF1A forms in JHH6 compared to HepG2, suggesting the possibility that eEF1A forms might become a relevant markers related to HCC tumor phenotype.
科研通智能强力驱动
Strongly Powered by AbleSci AI