胎盘
转录组
生物
Wnt信号通路
基因
合胞体
基因表达
核糖核酸
计算生物学
细胞生物学
生物信息学
胎儿
遗传学
怀孕
细胞
作者
Jessica Saben,Ying Zhang,Samantha S. McKelvey,Nafisa K. Dajani,Aline Andres,Thomas M. Badger,Horacio Gomez-Acevedo,Kartik Shankar
出处
期刊:Placenta
[Elsevier]
日期:2014-02-01
卷期号:35 (2): 125-131
被引量:54
标识
DOI:10.1016/j.placenta.2013.11.007
摘要
As the conduit for nutrients and growth signals, the placenta is critical to establishing an environment sufficient for fetal growth and development. To better understand the mechanisms regulating placental development and gene expression, we characterized the transcriptome of term placenta from 20 healthy women with uncomplicated pregnancies using RNA-seq. To identify genes that were highly expressed and unique to the placenta we compared placental RNA-seq data to data from 7 other tissues (adipose, breast, hear, kidney, liver, lung, and smooth muscle) and identified several genes novel to placental biology (QSOX1, DLG5, and SEMA7A). Semi-quantitative RT-PCR confirmed the RNA-seq results and immunohistochemistry indicated these proteins were highly expressed in the placental syncytium. Additionally, we mined our RNA-seq data to map the relative expression of key developmental gene families (Fox, Sox, Gata, Tead, and Wnt) within the placenta. We identified FOXO4, GATA3, and WNT7A to be amongst the highest expressed members of these families. Overall, these findings provide a new reference for understanding of placental transcriptome and can aid in the identification of novel pathways regulating placenta physiology that may be dysregulated in placental disease.
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