细胞生物学
神经炎症
化学
神经胶质
胶质纤维酸性蛋白
中枢神经系统
下调和上调
水通道蛋白
血脑屏障
作者
Masatoshi Ohnishi,Ayaka Monda,Ryoko Takemoto,Yukina Fujimoto,Mitsumasa Sugitani,Takahiro Iwamura,Takashi Hiroyasu,Atsuko Inoue
标识
DOI:10.1016/j.neuint.2014.05.007
摘要
To clarify the mechanism of high-mobility group box (HMGB) 1-induced brain edema formation, this study focused on the effect of HMGB1 on aquaporin (AQP) 4, a water channel, in rat brain. Treatments for 6h with 100-1000ng/ml HMGB1, not showing self-toxicity, of primary-cultured rat astrocytes didnot increase AQP4 mRNA, unexpectedly. In contrast, intracerebroventricular (i.c.v.) injection of 300ng of HMGB1 significantly increased AQP4 protein after 8h and formed edema after 24h in vivo. Thus, we investigated the roles of microglia as well as astrocytes. HMGB1 (1000ng/ml) drastically increased interleukin (IL)-1β in the primary-cultured rat microglia after 2h. The exposure of microglia to conditioned medium with HMGB1 and 3mM adenosine 5'-triphosphate for 6h significantly increased AQP4 mRNA in astrocytes after 6h. Although 1000ng/ml HMGB1 didnot induce transfer of nuclear factor (NF)-κB into the nucleus in astrocytes after 1h, the conditioned medium containing IL-1β led to its nuclear import. As factors likely to be involved in the nuclear import of NF-κB besides IL-1β, nitric oxide and tumor necrosis factor-α didnot contribute under these conditions. Finally, i.c.v. injection of 30nmol parthenolide, an NF-κB inhibitor, reversed 300ng of HMGB1 injection-induced AQP4 protein increase after 8h in vivo. The effect of parthenolide and the outcomes obtained so far suggest that HMGB1 indirectly up-regulates AQP4 expression through diffusible factor(s) such as IL-1β from microglia since HMGB1 by itself didnot affect NF-κB intracellular localization in astrocytes.
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