Neuroprotective effects of arachidonic acid against oxidative stress on rat hippocampal slices

花生四烯酸 生物化学 神经保护 谷氨酸受体 抗氧化剂 化学 药理学 谷胱甘肽 海马结构 过氧化氢酶 氧化应激 生物 内分泌学 受体
作者
Zejian Wang,Cui-Ling Liang,Guang-Mei Li,Cai-yi Yu,Ming Yin
出处
期刊:Chemico-Biological Interactions [Elsevier BV]
卷期号:163 (3): 207-217 被引量:123
标识
DOI:10.1016/j.cbi.2006.08.005
摘要

Arachidonic acid (AA), 5,8,11,14-eicosateraenoic acid is abundant, active and necessary in the human body. In the present study, we reported the neuroprotective effects and mechanism of arachidonic acid on hippocampal slices insulted by glutamate, NaN3 or H2O2in vitro. Different types of models of brain injury in vitro were developed by 1 mM glutamate, 10 mM NaN3 or 2 mM H2O2. After 30 min of preincubation with arachidonic acid or linoleic acid, hippocampal slices were subjected to glutamate, NaN3 or H2O2, then the tissue activities were evaluated by using the 2,3,5-triphenyltetrazolium chloride method. Endogenous antioxidant enzymes activities (SOD, GSH-PX and catalase) in hippocampal slices were evaluated during the course of incubation. MK886 (5 μM; a noncompetitive inhibitor of proliferator-activated receptor [PPAR]α), BADGE (bisphenol A diglycidyl ether; 100 μM; an antagonist of PPARγ) and cycloheximide (CHX; 30 μM; an inhibitor of protein synthesis) were tested for their effects on the neuroprotection afforded by arachidonic acid. Population spikes were recorded in randomly selected hippocapal slices. Arachidonic acid (1–10 μM) dose dependently protected hippocampal slices from glutamate and H2O2 injury (P < 0.01), and arachidonic acid (10 μM) can significantly improve the activities of Cu/Zn-SOD in hippocampal slices after 1 h incubation. In addition, 10 μM arachidonic acid significantly increased the activity of Mn-SOD and catalase, and decreased the activities of Cu/Zn-SOD to control value after 3 h incubation. These secondary changes of SOD during incubation can be reversed by indomethacine (10 μM; a nonspecific cyclooxygenase inhibitor) or AA 861 (20 μM; a 5-lipoxygenase inhibitor). Its neuroprotective effect was completely abolished by BADGE and CHX. These observations reveal that arachidonic acid can defense against oxidative stress by boosting the internal antioxidant system of hippocampal slices. Its neuroprotective effect may be mainly mediated by the activation of PPARγ and synthesis of new protein in tissue.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
昏睡的人完成签到,获得积分10
刚刚
刚刚
小韩完成签到,获得积分10
1秒前
1秒前
3秒前
谦让文昊完成签到,获得积分10
3秒前
3秒前
田安平完成签到 ,获得积分10
4秒前
火火完成签到,获得积分10
4秒前
磊磊发布了新的文献求助10
5秒前
自由平文完成签到 ,获得积分10
5秒前
欢喜念双完成签到,获得积分10
6秒前
7秒前
冲冲冲完成签到,获得积分10
7秒前
mushini完成签到,获得积分10
7秒前
lucia5354完成签到,获得积分10
7秒前
8秒前
听星伴月完成签到,获得积分10
8秒前
8秒前
9秒前
田...完成签到,获得积分10
10秒前
李爱国应助Beyond采纳,获得10
10秒前
xxx完成签到 ,获得积分10
10秒前
车车完成签到,获得积分10
11秒前
吴LY发布了新的文献求助10
11秒前
欢喜念双发布了新的文献求助10
12秒前
圆圆方方完成签到,获得积分10
12秒前
瀼瀼完成签到,获得积分10
14秒前
怡米李完成签到,获得积分10
14秒前
14秒前
木木发布了新的文献求助10
14秒前
弗兰奇将军完成签到,获得积分10
15秒前
15秒前
哈喽完成签到,获得积分10
15秒前
liudw完成签到,获得积分10
16秒前
tzy6665完成签到,获得积分10
16秒前
linwanshi完成签到,获得积分10
16秒前
聖璕完成签到,获得积分10
16秒前
沈归尘完成签到,获得积分10
17秒前
一川烟叶完成签到,获得积分10
18秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
徐淮辽南地区新元古代叠层石及生物地层 2000
A new approach to the extrapolation of accelerated life test data 1000
Global Eyelash Assessment scale (GEA) 500
School Psychology 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4030480
求助须知:如何正确求助?哪些是违规求助? 3569183
关于积分的说明 11356923
捐赠科研通 3299799
什么是DOI,文献DOI怎么找? 1816891
邀请新用户注册赠送积分活动 890975
科研通“疑难数据库(出版商)”最低求助积分说明 813983