环己醇
环己酮
己内酯
低聚物
化学
聚合
试剂
组合化学
单加氧酶
催化作用
有机化学
酶
细胞色素P450
聚合物
作者
Sandy Schmidt,Christian Scherkus,Jan Muschiol,Ulf Menyes,Till Winkler,Werner Hummel,Harald Gröger,Andreas Liese,Hans Georg Herz,Uwe T. Bornscheuer
标识
DOI:10.1002/anie.201410633
摘要
Abstract Poly‐ε‐caprolactone (PCL) is chemically produced on an industrial scale in spite of the need for hazardous peracetic acid as an oxidation reagent. Although Baeyer–Villiger monooxygenases (BVMO) in principle enable the enzymatic synthesis of ε‐caprolactone (ε‐CL) directly from cyclohexanone with molecular oxygen, current systems suffer from low productivity and are subject to substrate and product inhibition. The major limitations for such a biocatalytic route to produce this bulk chemical were overcome by combining an alcohol dehydrogenase with a BVMO to enable the efficient oxidation of cyclohexanol to ε‐CL. Key to success was a subsequent direct ring‐opening oligomerization of in situ formed ε‐CL in the aqueous phase by using lipase A from Candida antarctica, thus efficiently solving the product inhibition problem and leading to the formation of oligo‐ε‐CL at more than 20 g L −1 when starting from 200 m M cyclohexanol. This oligomer is easily chemically polymerized to PCL.
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