Formulation, characterization and evaluation of in vitro antioxidant potential of melatonin and quercetin loaded liposomes

The aim of the work was to use the thin film hydration process under vacuum condition to synthesize liposome loaded with melatonin and quercetin, as well as to improve the bioavailability of melatonin and quercetin from liposome.Phosphatidyl choline, cholesterol ratio, pH of hydration medium, rotating speed, and volume of solvent system were investigated as parameters impacting the entrapment of melatonin and quercetin liposome for formulation optimization.The result revealed that the  max of the melatonin and quercetin was at 276 and 372 nm, respectively.The size of optimized liposome formulation was 43.25 nm by FESEM having a zeta potential of -26.4 mV.Average particle sizes were found to be 1109 nm and PDI of 0.624.The drug entrapment efficiency was found to be 58.56% and 95.03% for melatonin and quercet, in respectively.The percentage drug release respectively for melatonin and quercetin was 68.1% and 79.5% in 24 h, respectively.The IC 50 value of melatonin and quercetin for DPPH inhibition was found to be 9.315µg/ml and 6.261 µg/ml, respectively and for synthesized liposome 5.64 µg/ml.Finally, the results concluded that the prepared liposome could be used as controlled drug delivery system that provided the better bioavailability and antioxidant activity as compared with the standard ascorbic acid, quercetin and melatonin.