微流控
单细胞分析
细胞
微流控芯片
实验室晶片
纳米技术
计算机科学
生化工程
计算生物学
生物
工程类
材料科学
生物化学
作者
Dian Anggraini,Noriyasu Ota,Yingjia Shen,Tao Tang,Yo Tanaka,Yoichiroh Hosokawa,Ming Li,Yaxiaer Yalikun
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:22 (8): 1438-1468
被引量:20
摘要
Single-cell analysis is essential to improve our understanding of cell functionality from cellular and subcellular aspects for diagnosis and therapy. Single-cell cultivation is one of the most important processes in single-cell analysis, which allows the monitoring of actual information of individual cells and provides sufficient single-cell clones and cell-derived products for further analysis. The microfluidic device is a fast-rising system that offers efficient, effective, and sensitive single-cell cultivation and real-time single-cell analysis conducted either on-chip or off-chip. Here, we introduce the importance of single-cell cultivation from the aspects of cellular and subcellular studies. We highlight the materials and structures utilized in microfluidic devices for single-cell cultivation. We further discuss biological applications utilizing single-cell cultivation-based microfluidics, such as cellular phenotyping, cell-cell interactions, and omics profiling. Finally, present limitations and future prospects of microfluidics for single-cell cultivation are also discussed.
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