内科学
胰岛素抵抗
内分泌学
脂肪生成
CD36
脂质代谢
葡萄糖稳态
炎症
磷酸化
医学
平衡
碳水化合物代谢
胰岛素
生物
化学
生物化学
受体
作者
Gabriel Calheiros Antunes,Robson Damasceno de Lima,Renan Fudoli Lins Vieira,Ana Paula Azevêdo Macêdo,Vitor Rosetto Muñoz,Erika Pereira Zambalde,Caio Felipe Romeiro,Fernando Moreira Simabuco,Patricia Oliveira Prada,Adelino Sanchez Ramos da Silva,Eduardo Rochete Ropelle,Dennys Esper Cintra,José Rodrigo Pauli
标识
DOI:10.1111/1440-1681.13687
摘要
Obesity is associated with low-grade inflammation and disturbances in hepatic metabolism. This study aimed to investigate the effects of resistance exercise on inflammatory signalling related to IκB kinase (IKK) ɛ protein (IKKɛ) and on hepatic fat accumulation in obese mice. Male Swiss mice were distributed into three groups: control (CTL) fed with standard chow; obese (OB) mice induced by a high-fat diet (HFD); obese exercised (OB + RE) mice fed with HFD and submitted to a resistance exercise training. The resistance exercise training protocol consisted of 20 sets/3 ladder climbs for 8 weeks, three times/week on alternate days. The training overload was equivalent to 70% of the maximum load supported by the rodent. Assays were performed to evaluate weight gain, hepatic fat content, fasting glucose, insulin sensitivity, IKKɛ phosphorylation and proteins related to insulin signalling and lipogenesis in the liver. Mice that received the high-fat diet showed greater adiposity, impaired insulin sensitivity, increased fasting glucose and increased hepatic fat accumulation. These results were accompanied by an increase in IKKɛ phosphorylation and lipogenesis-related proteins such as cluster of differentiation 36 (CD36) and fatty acid synthase (FAS) in the liver of obese mice. In contrast, exercised mice showed lower body weight and adiposity evolution throughout the experiment. In addition, resistance exercise suppressed the effects of the high-fat diet by reducing IKKɛ phosphorylation and hepatic fat content. In conclusion, resistance exercise training improves hepatic fat metabolism and glycaemic homeostasis, which are, at least in part, linked to the anti-inflammatory effect of reduced IKKɛ phosphorylation in the liver of obese mice.
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