WRKY蛋白质结构域
生物
胡桃
基因
基因表达
植物抗病性
MYB公司
基因沉默
转录组
基因表达调控
转录因子
遗传学
植物
作者
Rui Zhou,Yuhui Dong,Xia Liu,Shan Feng,Changxi Wang,Xin-Mei Ma,Jian-Ning Liu,Qiang Liang,Yan Bao,Shengyi Xu,Xinya Lang,Shasha Gai,Ke Yang,Hongcheng Fang
出处
期刊:Plant Journal
[Wiley]
日期:2022-07-20
卷期号:111 (4): 1152-1166
被引量:7
摘要
Walnut (Juglans regia L.) anthracnose induced by Colletotrichum gloeosporioides is the catastrophic disease impacting walnut industry in China. Although WRKY transcription factors play a key role in plant immunity, the function of WRKY gene in walnut resistance to C. gloeosporioides is not clear. Here, we identified a differentially expressed gene, JrWRKY21, through transcriptome sequencing and qRT-PCR that was significantly upregulated upon C. gloeosporioides infection in walnut. JrWRKY21 positively regulated the walnut resistance to C. gloeosporioides as demonstrated in virus-induced gene silencing and transient gene overexpression. Additionally, JrWRKY21 directly interacted with the transcriptional activator of PR gene JrPTI5L in vitro and in vivo and could bind to the W-box in the JrPTI5L promoter for transcriptional activation. And JrPTI5L could induce the expression of PR gene JrPR5L through binding GCCGAC motif in promoter. Our data support that JrWRKY21 can indirectly activate the expression of JrPR5L gene by WRKY21-PTI5L protein complex to promote resistance against C.gloeosporioides in walnut. The results will enhance our understanding of the walnut disease resistance mechanism and facilitate the genetic improvement of walnut by the molecular design breeding for anthracnose resistance varieties.
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