Facile Synthesis of Fluorescent Hyper-Cross-Linked β-Cyclodextrin-Carbon Quantum Dot Hybrid Nanosponges for Tumor Theranostic Application with Enhanced Antitumor Efficacy

Fluorescent hyper-cross-linked β-cyclodextrin-carbon quantum dot (β-CD-CQD) hybrid nanosponges of about 200 nm with excellent biocompatibility and strong bright blue fluorescence excited at 365 nm with a high photoluminescence quantum yield (PLQY) of 38.0% were synthesized for tumor theranostic application by facile condensation polymerization of carbon quantum dots (CQDs) with β-cyclodextrin (β-CD) at a feeding ratio of 1:5. The DOX@β-CD-CQD theranostic nanomedicine, around 300 nm with DOX-loading capacity of 39.5% by loading doxorubicin (DOX) via host-guest complexation, showed a pH responsive controlled release and released DOX in the simulated tumor microenvironment in a sustained release mode, owing to the formation constant in the supramolecular complexation of DOX with the β-CD units in the β-CD-CQD nanosponges. The proposed DOX@β-CD-CQD theranostic nanomedicine could be internalized into HepG2 cells, and the released DOX was accumulated into the cell nuclei, demonstrating an antitumor efficacy more enhanced than that of the free drug.