Synergism of organic acid and deep eutectic solvents pretreatment for the co-production of oligosaccharides and enhancing enzymatic saccharification

A novel pretreatment using organic acid synergism with deep eutectic solvents (DESs) was developed to the co-production of oligosaccharides, especially for the functional oligosaccharides, and enhancement of corn straws enzymatic saccharification. It was found that lactic acid (Lac) pretreatment combined with choline chloride/Lac system could not only selectively convert the hemicellulose to xylo-oligosaccharides (XOS), which account for 89.7% of total xylose in prehydrolysate (the functional XOS (DP < 5) took up about 35%), but also significantly promote the glucose release (33.2 g/100 g material) and well lignin separation (representing 40.9% in whole process), which better than the single organic pretreatment at higher modified severity index (SI). Structural features of various solids were characterized to better comprehend how hemicellulose and lignin removal influenced enzymatic hydrolysis. This work offered the mild and potential method to co-produce fermentable sugars with the effective separation and valorization of lignin.