雷米普利
医学
内科学
血管紧张素转化酶2
内分泌学
血管紧张素II
肾素-血管紧张素系统
收缩性
心肌细胞
血管紧张素转换酶抑制剂
心肌梗塞
肌酸激酶
肌肉肥大
血管紧张素转换酶
血压
传染病(医学专业)
疾病
2019年冠状病毒病(COVID-19)
作者
Louise M. Burrell,John Risvanis,Eiji Kubota,Rachael Dean,P. Macdonald,Lucy Lu,Christos Tikellis,Sharon Grant,Rebecca A. Lew,A. Ian Smith,Mark E. Cooper,Colin I. Johnston
标识
DOI:10.1093/eurheartj/ehi114
摘要
Angiotensin converting enzyme (ACE) 2 catalyses the cleavage of angiotensin (Ang) I to Ang 1-9 and of Ang II to Ang 1-7. ACE2 deficiency impairs cardiac contractility and upregulates hypoxia-induced genes, suggesting a link with myocardial ischaemia. We studied the expression of ACE2 after myocardial infarction (MI) in the rat as well as in human failing hearts.Rats were killed at days 1, 3, and 28 after MI, or treated for 4 weeks with the ACE inhibitor ramipril (1 mg/kg). Cardiac gene and protein expression of ACE and ACE2 were assessed by quantitative real-time reverse transcriptase-polymerase chain reaction and immunohistochemistry/activity assays/in vitro autoradiography, respectively. Both ACE (P = 0.022) and ACE2 (P = 0.015) mRNA increased in the border/infarct area compared with the viable area at day 3 after MI. By day 28, increases in ACE (P = 0.005) and ACE2 (P = 0.006) mRNA were also seen in the viable myocardium of MI rats compared with myocardium of control rats. ACE2 protein localized to macrophages, vascular endothelium, smooth muscle, and myocytes. Ramipril attenuated cardiac hypertrophy and inhibited cardiac ACE. In contrast, ramipril had no effect on cardiac ACE2 mRNA, which remained elevated in all areas of the MI rat heart. Immunoreactivity of both ACE and ACE2 increased in failing human hearts.The increase in ACE2 after MI suggests that it plays an important role in the negative modulation of the renin angiotensin system in the generation and degradation of angiotensin peptides after cardiac injury.
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