胶质肉瘤
间充质干细胞
胶质纤维酸性蛋白
生物
病理
比较基因组杂交
癌症研究
分子生物学
免疫组织化学
基因
基因组
胶质母细胞瘤
遗传学
免疫学
医学
作者
Masaya Nagaishi,Young Ho Kim,Michel Mittelbronn,Felice Giangaspero,Werner Paulus,Benjamin Brokinkel,Anne Vital,Yūko Tanaka,Yoichi Nakazato,Catherine Legras-Lachuer,Joël Lachuer,Hideaki Ohgaki
标识
DOI:10.1016/j.ajpath.2012.01.027
摘要
Gliosarcoma is a rare glioblastoma variant characterized by a biphasic tissue pattern with alternating areas that display either glial (glial fibrillary acidic protein-positive) or mesenchymal (reticulin-positive) differentiation. Previous analyses have shown identical genetic alterations in glial and mesenchymal tumor areas, suggesting that gliosarcomas are genetically monoclonal, and mesenchymal differentiation was considered to reflect the elevated genomic instability of glioblastomas. In the present study, we compared genome-wide chromosomal imbalances using array comparative genomic hybridization in glial and mesenchymal tumor areas of 13 gliosarcomas. The patterns of gain and loss were similar, except that the gain at 13q13.3-q14.1 (log(2) ratio >3.0), containing the STOML3, FREM2, and LHFP genes, which was restricted to the mesenchymal tumor area of a gliosarcoma. Further analyses of 64 cases of gliosarcoma using quantitative PCR showed amplification of the STOML3, FREM2, and LHFP genes in 14 (22%), 10 (16%), and 7 (11%) mesenchymal tumor areas, respectively, but not in glial tumor areas. Results of IHC analysis confirmed that overexpression of STOML3 and FREM2 was more extensive in mesenchymal than in glial tumor areas. These results suggest that the mesenchymal components in a small fraction of gliosarcomas may be derived from glial cells with additional genetic alterations.
科研通智能强力驱动
Strongly Powered by AbleSci AI