One strain of bacterium that is capable of using acetaminophen as sole source of carbon and energy was isolated and characterized.This strain was obtained by sludge from Shangtang River of Hangzhou with compounds like acetaminophen.Strain PAR-9 was identified as Pseudomonas genus,via analyzing Physiological and biochemical characters,the morphological characteristics and 16S rDNA sequence homologue.analysis identified.Strain PAR-9 degraded 96% of 1 500 mg/L acetaminophen in 12 h.Conditions for the complete biodegradation of acetaminophen were optimized.Optimum growth temperature and pH were found to be 30 ℃ and 6.5~7,respectively.Strain PAR-9 was an efficient acetaminophen degradation strain,which can be adopted to deal with wastewater containing acetaminophen.The discovery of strain PAR-9 increased the acetaminophen degrading bacteria library which currently has very low bacterial diversity.