亚甲蓝
生物传感器
检出限
DNA
适体
化学
电化学
纳米技术
滚动圆复制
组合化学
生物物理学
分子生物学
电极
材料科学
色谱法
生物化学
DNA复制
生物
催化作用
物理化学
光催化
作者
Jingsi Yang,Qizhi Liang,Quan Zhou,Lingjun Sha,Hai Shi,Genxi Li
标识
DOI:10.1016/j.bios.2023.115907
摘要
Human trophoblast surface cell antigen 2 (Trop-2) on the tumor cell membrane can not only serve as the target for chemotherapy drugs, but also as a biomarker for typing and prognosis of breast cancer; however, assay of Trop-2 is seriously hampered due to the limitations of available tool. Herein, we have designed and fabricated an electrochemical biosensor for the assay of Trop-2 based on methylene blue (MB)-assisted assembly of DNA nanocomposite particles (DNPs). Specially, the recognition between Trop-2 and its aptamer may activate the primer exchange reaction (PER) on an electrode surface to produce long single-strand DNA (ssDNA) which can be self-assembled into DNPs by electrostatic interaction between negative charged DNA and positive charged and electro-active MB molecules which can also be used to give electrochemical signal. By using this electrochemical biosensor, ultrasensitive detection of tumor cells with high Trop-2 expressions can be conducted, with the limit of detection (LOD) of 1 cell/mL. Moreover, this biosensor can be further used for accurately profiling Trop-2 expression of tumor cells in mouse tissues, suggesting its great potential in the precise definition of breast cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI