Cell factories for methylerythritol phosphate pathway mediated terpenoid biosynthesis: An application of modern engineering towards sustainability

Terpenoids and their derivatives which are synthesized either through the methylerythritol phosphate (MEP) or mevalonate (MVA) pathways represent a most diversified class of natural compounds with a wide range of therapeutic properties such as anticancer, antimicrobial, antiparasitic, and antiallergenic. Globally, terpenoids are in high demand due to their excellent pharmaceutical applications and their potential benefits in many sectors. Since tiny quantities of these compounds are present in nature, their large-scale uses are limited. Moreover, the petroleum-based production of these compounds is managing the current market demand worldwide. Nevertheless, the increase in greenhouse gas emissions and global warming makes the above approach unsustainable for commercial scale production. This can be addressed by metabolic pathway engineering, a promising method of synthetic biology that enables the efficient modification of microbial pathways to produce high value compounds on a continuous and sustainable basis. In this review, we primarily focused on the importance and utility of the MEP pathway toward the biosynthesis of specific terpenoids. In addition, different types of terpenoids, their industrial values, and engineering initiatives to increase the titers of valuable terpenoids from promising cell factories are also discussed.