Near-Infrared Fluorescent Probe for Imaging Upregulated Hydrogen Sulfide Levels in Rice under Salt and Drought Stress

Hydrogen sulfide (H₂S) is a hazardous gas found in living organisms and is directly tied to our daily lives. Recent studies show that it plays a significant role in plant growth, development, and response to environmental stresses. However, few of the reported near-infrared (NIR) fluorescent probes have been applied to rice and deeply investigated the influence of the external environment on the biological molecules in its internal environment. Therefore, our team created BSZ-H₂S, which has the advantage of an emission wavelength of up to 720 nm with fast response, successfully applying it to cell and zebrafish imaging. More importantly, the probe detected H₂S in rice roots by in situ imaging in a facile manner and verified the existence of an upregulation process of H₂S in response to salt and drought stress. This work provides a concept for the intervention of external stresses in rice culture.