Introducing uracil-containing oligo to improve exponential amplification reaction performance in biosensor application

Exponential amplification reaction (EXPAR) has the advantages of high detection sensitivity and short detection time, but it also has the problem of high background signal. In this paper, we innovatively added the uracil-containing oligo that could inhibit the non-specific activity of Vent (exo-) DNA polymerase to increase the detection effect of EXPAR.